FastQCFastQC Report
Tue 17 May 2016
G128-M13-B_AGTTCC_AC8WFRANXX_L001_001.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG128-M13-B_AGTTCC_AC8WFRANXX_L001_001.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16504883
Sequences flagged as poor quality0
Sequence length50
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG1918641.1624681011068057Illumina Single End PCR Primer 1 (100% over 50bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA458070.27753604796834974No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT406630.2463695137978258No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA260050.041.8159141
CGTGTAG256950.041.7893315
TCGTGTA258700.041.71072814
AGAGCGT265800.041.548628
GTCGTGT268600.041.4019113
CGTCGTG266650.041.25104512
TCTCGGT261800.040.67075736
GCGTCGT271950.040.64946711
GGTCGCC256750.040.4689843
GTGGTCG261050.040.4536141
ATCGGAA268450.040.212452
GTGTAGG271450.040.0515616
GTAGATC263550.040.0075331
TGGTCGC262050.039.99095542
GTAGGGA260050.039.8532818
CTCGGTG265600.039.7658437
GAGCGTC275250.039.722539
CGGTGGT269100.039.71774339
AGCGTCG276850.039.68367410
TGTAGGG275250.039.57055717
Produced by FastQC (version 0.11.2)