FastQCFastQC Report
Tue 17 May 2016
G128-M12-B_CTTGTA_AC8WFRANXX_L001_001.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG128-M12-B_CTTGTA_AC8WFRANXX_L001_001.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21116602
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGC1460480.6916264273958471TruSeq Adapter, Index 12 (100% over 50bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT219500.10394664823440818No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA181500.041.62811
CGTATGC181350.040.56551444
TCGTATG183400.040.4119743
CTCGTAT181500.039.79261842
ATCGGAA190250.039.4989972
ACACGTC187200.039.355213
CGTCTGA189500.039.17936716
ACGTCTG192900.038.54582215
TCTCGTA184200.038.03891441
CACGTCT196700.037.58867614
CACACGT197000.037.45326612
AATCTCG193950.035.95567739
ATCTCGT195300.035.91048440
CGGAAGA216200.034.737674
TCGGAAG217300.034.6731833
GCACACG216750.034.66982311
GTAATCT204850.034.1185537
TAATCTC206900.033.7483638
ACCTTGT221850.033.18333432
CTTGTAA223050.032.7561234
Produced by FastQC (version 0.11.2)