FastQCFastQC Report
Tue 17 May 2016
G128-M11-B_GGCTAC_AC8WFRANXX_L001_001.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG128-M11-B_GGCTAC_AC8WFRANXX_L001_001.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11126814
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATGC4408633.9621674272617478TruSeq Adapter, Index 11 (100% over 50bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT503700.45269023100413114No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA515450.043.584581
ATCGGAA521000.042.907792
CGTATGC519200.042.85008644
ACACGTC518600.042.81366713
ACGTCTG521250.042.75216315
TCGTATG523700.042.7171343
CGTCTGA521800.042.707116
ACGGCTA520750.042.69758232
CTCGTAT516800.042.53400842
CACACGT523750.042.51029212
CGGCTAC522300.042.4649833
CACGTCT523900.042.4267414
TCACGGC526000.042.16684730
CGGAAGA528300.042.0858734
TCTCGTA503600.042.03694541
CACGGCT529350.041.98311631
GTCACGG534800.041.64784229
AGTCACG535450.041.64621728
CATCTCG509800.041.43490239
TCGGAAG540050.041.4146083
Produced by FastQC (version 0.11.2)