FastQCFastQC Report
Tue 17 May 2016
G128-M1-B_ATCACG_AC8WFRANXX_L001_001.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG128-M1-B_ATCACG_AC8WFRANXX_L001_001.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10221845
Sequences flagged as poor quality0
Sequence length50
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG1374501.3446691864335647Illumina Single End PCR Primer 1 (100% over 50bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT261120.2554529050283975No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTGTA179050.042.3885314
GATCGGA179200.042.2067681
AGAGCGT184850.041.93968
CGTGTAG180550.041.8655815
GTCGTGT187200.041.81226713
ATCGGAA180750.041.577062
TCTCGGT183200.041.2249836
GATCTCG181400.041.18512334
GTGGTCG181200.041.0734741
ATCTCGG182000.040.91658835
AAGAGCG185100.040.908187
GGTCGCC178200.040.90530443
GTAGATC185250.040.62408431
TCGGTGG189600.040.43836238
TCGGAAG188450.040.181763
GTAGGGA180900.040.15542618
TGTAGGG191400.040.05571417
CTCGGTG186800.040.0300737
CGGTGGT190350.039.93464339
GAGTGTA194500.039.6193327
Produced by FastQC (version 0.11.2)