FastQCFastQC Report
Tue 17 May 2016
G128-M1-B_ATCACG_AC8WFRANXX_L001_001.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG128-M1-B_ATCACG_AC8WFRANXX_L001_001.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10221845
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGC1590611.5560889448039956TruSeq Adapter, Index 1 (100% over 50bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT133090.13020154385044969No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA193350.042.7422031
CGTATGC190550.042.2551744
ACACGTC192500.041.96303613
TCGTATG193650.041.87410743
CGTCTGA192450.041.8139116
CTCGTAT188300.041.79038242
ACGTCTG193350.041.7671815
ACGATCT179800.041.47758537
TCACGAT183600.041.30310435
CACGTCT196600.041.13267514
CACACGT196900.041.11469312
CACGATC181150.040.926436
TCGGAAG202050.040.6982233
CGGAAGA201050.040.6599274
ATCACGA197350.040.59974334
CGATCTC185550.040.38233638
ATCGGAA204700.040.063882
GATCTCG187500.040.0442939
TCTCGTA189050.039.92562541
CATCACG201600.039.84420433
Produced by FastQC (version 0.11.2)