FastQCFastQC Report
Wed 4 May 2016
G131-M9_GTTTCG_BC8GTYANXX_L002_001.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG131-M9_GTTTCG_BC8GTYANXX_L002_001.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11455570
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG637600.5565851371865389Illumina Single End PCR Primer 1 (100% over 50bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT450600.39334576978709923No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA194650.16991734152032592No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCTCG79850.037.78867734
ATCTCGG80650.037.3860835
TCTCGGT81300.037.33161536
GTAGATC81600.037.0357331
TCGTGTA80950.036.6634514
GCGTCGT83700.035.88099311
CGTGTAG83650.035.60579715
CGTCGTG84400.035.5829312
GTCGTGT84750.035.38390713
GTCGCCG85900.035.0807444
TGGTCGC87550.034.5175342
GTAGGGA86800.034.5147318
AGAGCGT87550.034.4835628
GAGTGTA88050.034.35220327
GATCGGA87950.034.2021941
GGTCGCC88500.034.0480443
TAGATCT89200.033.92745632
ATCGGAA88600.033.7775882
AGCGTCG89250.033.67556410
TGTAGGG90000.033.55696517
Produced by FastQC (version 0.11.2)