FastQCFastQC Report
Wed 4 May 2016
G131-M8_GTGGCC_BC8GTYANXX_L002_001.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG131-M8_GTGGCC_BC8GTYANXX_L002_001.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19778183
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG634570.3208434263147429Illumina Single End PCR Primer 1 (100% over 50bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT326910.1652881864830556No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCTCG88450.034.13997734
GCGTCGT90500.033.81614711
GTAGATC91600.033.3769131
TCGTGTA90450.033.22505614
TCTCGGT91900.033.2170636
CGTCGTG92750.033.11416212
ATCTCGG92200.032.60796735
CGTGTAG93550.032.21486715
AGCGTCG96050.032.11511210
GATCGGA95750.031.7825661
GTCGTGT96800.031.52343213
GTAGGGA95700.031.51220118
ATCGGAA98150.030.8262392
AGAGCGT99750.030.8173338
GTCGCCG98400.030.69208744
GAGCGTC101950.030.3001849
TGGTCGC100500.030.20129242
GTGTAGG103050.029.54314216
TGTAGGG103200.029.41434717
GTGGTCG103700.029.39659141
Produced by FastQC (version 0.11.2)