FastQCFastQC Report
Wed 4 May 2016
G131-M7_GTGAAA_BC8GTYANXX_L002_001.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG131-M7_GTGAAA_BC8GTYANXX_L002_001.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14852273
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG902870.6079002183706157Illumina Single End PCR Primer 1 (100% over 50bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT880640.5929328123715475No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCTCG115100.037.92741834
GTAGATC115000.037.92572831
ATCTCGG115750.037.6003135
GTCGCCG115150.037.4399244
TCTCGGT116250.037.4008836
GCGTCGT116050.037.14175411
TCGTGTA115750.037.08357214
CGTGTAG117900.036.5876415
TGGTCGC119650.036.15720742
CGTCGTG119100.036.15328612
AGCGTCG120200.036.0616110
GTAGGGA120900.035.62192518
GGTCGCC121500.035.57069843
ATCGGAA121450.035.468282
GATCGGA122550.035.400551
GTCGTGT121600.035.37357713
GAGTGTA123800.035.146527
GTGGTCG123400.035.129641
AGAGCGT124800.034.7726068
GAGCGTC125800.034.5267529
Produced by FastQC (version 0.11.2)