FastQCFastQC Report
Wed 4 May 2016
G131-M6_GTCCGC_BC8GTYANXX_L002_001.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG131-M6_GTCCGC_BC8GTYANXX_L002_001.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12287457
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCACATCTCGTAT468770.3815028610069602TruSeq Adapter, Index 18 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCCG67150.040.77812232
GATCGGA69700.040.1858671
CGTCCGC71150.038.5292233
ATCGGAA73400.038.0334552
ACACGTC74650.037.23797613
CGTCTGA77050.036.62152516
GTCCGCA73850.036.48084334
ACGTCTG77700.036.28949715
AGTCACG76250.036.20088228
GTCACGT76850.035.96533629
CTCGTAT72100.035.8535144
TCACGTC78500.035.0530130
CACACGT80700.034.66686612
CGGAAGA81300.034.4250684
CGCACAT69350.034.4022137
CACGTCT82800.034.1072114
TCCGCAC80000.033.84187735
TCTCGTA68700.033.3740243
CACGTCC84050.033.00323531
GCACACG86000.032.65972511
Produced by FastQC (version 0.11.2)