FastQCFastQC Report
Wed 4 May 2016
G131-M4_ATGTCA_BC8GTYANXX_L002_001.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG131-M4_ATGTCA_BC8GTYANXX_L002_001.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14629393
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGTAT795030.5434470179316394TruSeq Adapter, Index 15 (97% over 40bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA240020.16406695752858644No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA119850.040.9246641
ATCGGAA124500.039.32062
ACACGTC125100.038.8756113
CGTCTGA126450.038.51235216
ACGTCTG127750.038.48721315
AATCTCG107150.038.36666541
CTCGTAT119500.038.0760644
CGGAAGA133150.036.8880654
CACACGT132750.036.44301212
CACGTCT135150.036.4420814
TCTCGTA115700.035.9271443
TCGGAAG139050.035.4600753
ATCTCGT120750.034.3547242
GCACACG141300.034.1932411
ACATGTC154450.031.72814232
GAATCTC131450.031.359940
TCTGAAC154600.030.97282218
ATGTCAG159050.030.58516734
GAACTCC155750.030.54859721
CACATGT162350.030.36602231
Produced by FastQC (version 0.11.2)