FastQCFastQC Report
Wed 4 May 2016
G131-M2_CGATGT_BC8GTYANXX_L002_001.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG131-M2_CGATGT_BC8GTYANXX_L002_001.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13306075
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGC272320.20465839851346096TruSeq Adapter, Index 2 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA42650.037.6526761
CGTATGC43450.035.61734844
TCGTATG45400.034.52408243
TCACCGA46250.033.97671530
ATCGGAA47400.033.7792742
ACGTCTG49800.032.37635415
ACACGTC49100.032.16150313
CGATGTA48400.031.71576334
CCGATGT49250.031.37853433
CGTCTGA51050.031.17388216
ACCGATG50400.030.91622532
CTCGTAT48450.030.7637442
GTCACCG52400.030.41486729
CACCGAT51700.030.3981231
TATCTCG45500.029.83148839
CACACGT54300.029.3879912
CACGTCT55350.029.36760514
CGGAAGA57350.028.4589314
TCTCGTA50050.027.27693741
GATGTAT52650.026.48231935
Produced by FastQC (version 0.11.2)