FastQCFastQC Report
Wed 4 May 2016
G131-M14_AGTCAA_BC8GTYANXX_L002_001.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG131-M14_AGTCAA_BC8GTYANXX_L002_001.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10296694
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG577630.5609858853725282Illumina Single End PCR Primer 1 (100% over 50bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT301850.2931523457917658No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGATC74550.037.1738331
GATCTCG75150.036.93359434
TCGTGTA75150.036.4766914
TCTCGGT77400.035.91642436
GCGTCGT77700.035.8187711
GTCGCCG76950.035.81694844
ATCTCGG77700.035.6358835
CGTGTAG78150.035.21359315
CGTCGTG79950.034.83774612
GTAGGGA78150.034.78950518
GTCGTGT80450.034.56653213
TGGTCGC80400.034.4960442
GAGTGTA80500.034.40406427
GGTCGCC80200.034.3628443
ATCGGAA81450.033.958432
AGCGTCG82350.033.7434410
GATCGGA82350.033.5873031
AGTGTAG83300.033.2719328
TGTAGGG82950.033.17478617
TAGGGAA82250.033.08222619
Produced by FastQC (version 0.11.2)