FastQCFastQC Report
Wed 4 May 2016
G131-M11_GAGTGG_BC8GTYANXX_L002_001.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG131-M11_GAGTGG_BC8GTYANXX_L002_001.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7857957
Sequences flagged as poor quality0
Sequence length50
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG595110.7573342536743329Illumina Single End PCR Primer 1 (100% over 50bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT524290.6672090468298567No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA124860.1588962627308854No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCTCG72000.039.34210234
GTCGCCG71900.039.31081844
TGGTCGC72150.039.08040642
TCTCGGT72750.038.93651236
ATCTCGG72750.038.93626435
GCGTCGT73000.038.78713211
GTAGATC73850.038.74665531
TCGTGTA72400.038.68166714
GGTCGCC73600.038.48978443
CGTCGTG73750.038.3029512
AGCGTCG74700.038.2303510
GTGGTCG74750.037.80936441
CGTGTAG74200.037.7970615
ATCGGAA75000.037.6772652
GTCGTGT75500.037.44355813
GTAGGGA75250.037.12137218
GATCGGA76650.036.952181
GAGCGTC77500.036.821679
CTCGGTG77200.036.806337
AGAGCGT77350.036.7849248
Produced by FastQC (version 0.11.2)