FastQCFastQC Report
Wed 4 May 2016
G131-M10_CGTACG_BC8GTYANXX_L002_001.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG131-M10_CGTACG_BC8GTYANXX_L002_001.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8416513
Sequences flagged as poor quality0
Sequence length50
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTACGTAATCTCGTAT695360.8261853810479471TruSeq Adapter, Index 22 (97% over 40bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA493530.586382983071493No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTACGT96350.042.22667734
CCGTACG96650.042.2152933
GATCGGA99600.042.124461
ACCGTAC97150.042.0991332
GTACGTA96350.041.70194235
ATCGGAA101000.041.4916272
CGTAATC82150.041.4147538
CACCGTA99050.041.3867331
ACGTCTG102300.040.86462415
ACACGTC101350.040.7480713
CTCGTAT92450.040.72793244
CGTCTGA102950.040.593616
TACGTAA98450.040.56572336
GTCACCG103150.040.1387829
AATCTCG84750.039.99633441
ACGTAAT89300.039.92314537
CGGAAGA105150.039.6076434
TCACCGT104750.039.5927730
CACGTCT105550.039.583614
CACACGT105150.039.20786312
Produced by FastQC (version 0.11.2)