FastQCFastQC Report
Wed 4 May 2016
G128-M9_GATCAG_BC8GTYANXX_L001_001.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG128-M9_GATCAG_BC8GTYANXX_L001_001.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17156077
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG2056321.1985956929430894Illumina Single End PCR Primer 1 (100% over 50bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA334670.19507373393113123No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN216030.12592039543772157No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCTCG248600.041.19228734
TCTCGGT252050.040.74201636
ATCTCGG251700.040.64150235
TCGTGTA247300.040.51244714
GTAGATC252950.040.4532631
CGTGTAG250850.040.23718615
GTGGTCG254400.039.98538241
GATCGGA254000.039.9808771
GTCGTGT255050.039.7121313
CGTCGTG254500.039.68604312
GCGTCGT254400.039.66199511
AGAGCGT258500.039.4225048
GTAGGGA254950.039.38369818
ATCGGAA256450.039.3584482
GAGTGTA260700.039.30325327
TGGTCGC257850.039.1173842
GTGTAGG260750.038.8445916
AGTGTAG264750.038.73343728
TCGGTGG267200.038.69617538
GGTCGCC260350.038.6249543
Produced by FastQC (version 0.11.2)