FastQCFastQC Report
Wed 4 May 2016
G128-M8_ACTTGA_BC8GTYANXX_L001_001.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG128-M8_ACTTGA_BC8GTYANXX_L001_001.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13724172
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG1695931.235724821869035Illumina Single End PCR Primer 1 (100% over 50bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT472130.3440134676248593No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA207140.15093078110650318No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN165110.12030598275801264No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTGTA200600.041.16056414
CGTGTAG201800.040.95746615
GATCTCG205000.040.8666234
ATCTCGG206100.040.61678735
GATCGGA207500.040.4401471
TCTCGGT209250.040.40492236
GTAGATC207850.040.4038631
GTCGTGT209150.039.9609313
AGAGCGT212400.039.8886458
ATCGGAA210200.039.7844122
GTAGGGA208650.039.62395518
GTGGTCG210900.039.47321341
TGTAGGG210550.039.4441617
GCGTCGT213350.039.3428411
GGTCGCC210200.039.2704143
CGTCGTG213600.039.22196612
GTGTAGG212950.039.09259416
AAGAGCG217650.038.9815947
GAGTGTA216750.038.81898527
CGGTGGT216850.038.7755639
Produced by FastQC (version 0.11.2)