FastQCFastQC Report
Wed 4 May 2016
G128-M8_ACTTGA_BC8GTYANXX_L001_001.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG128-M8_ACTTGA_BC8GTYANXX_L001_001.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13724172
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGC1410621.027836141954502TruSeq Adapter, Index 8 (100% over 50bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA390940.2848550717668068No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT304390.22179115796566815No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA191050.042.3986851
TCGTATG192250.041.673943
CGTATGC192500.041.45962544
CTCGTAT185450.041.30758342
CGTCTGA198100.041.2492516
ACGTCTG201000.041.0472115
ACACGTC200700.040.98460813
ATCGGAA199750.040.4755442
CACGTCT204200.040.3927514
CACACGT203050.040.15146312
TCTCGTA180600.039.40173741
CGGAAGA204700.039.2180634
TCGGAAG211650.038.4941443
TTGAATC189500.037.20329336
GCACACG221300.036.8491511
GAACTCC221150.036.81663521
ATCTCGT194300.036.56714640
CACTTGA221250.035.80616833
CTGAACT229150.035.77144619
TGAATCT197400.035.6771637
Produced by FastQC (version 0.11.2)