FastQCFastQC Report
Wed 4 May 2016
G128-M5A_ACAGTG_BC8GTYANXX_L001_001.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG128-M5A_ACAGTG_BC8GTYANXX_L001_001.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12138023
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG4128553.4013364449877876Illumina Single End PCR Primer 1 (100% over 50bp)
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN144150.1187590433796344No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGATC476200.042.5105531
GATCTCG475150.042.43948734
GCGTCGT473950.042.36408611
TCGTGTA469950.042.3471714
TCTCGGT478400.042.30714436
ATCTCGG476450.042.30487435
CGTGTAG472450.042.289515
GTAGGGA471100.042.22442218
CGTCGTG475950.042.1646212
TGGTCGC477200.042.05932242
GAGTGTA480200.041.9841127
GTCGCCG478750.041.96822444
GTGGTCG479650.041.9591341
GTCGTGT478100.041.94687713
AGCGTCG479700.041.92681510
AGTGTAG481700.041.8519928
GGTCGCC480950.041.72447643
TGTAGGG480500.041.66393317
TAGGGAA474800.041.64519
GATCGGA484450.041.630181
Produced by FastQC (version 0.11.2)