G128-M13_AGTTCC_BC8GTYANXX_L001_001.R2.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	G128-M13_AGTTCC_BC8GTYANXX_L001_001.R2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	18646381
Sequences flagged as poor quality	0
Sequence length	50
%GC	46

Per base quality graph

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG	270181	1.4489728596664415	Illumina Single End PCR Primer 1 (100% over 50bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	78991	0.42362643989737203	No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	37199	0.19949715711590363	No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN	23223	0.12454427483810397	No Hit

Adapter graph

Kmer graph

Sequence	Count	Obs/Exp Max	Max Obs/Exp Position
CGTGTAG	31815	41.619167	15
TCGTGTA	31790	41.548992	14
GTCGTGT	32260	41.29882	13
CGTCGTG	32360	41.1518	12
TCTCGGT	32410	41.077824	36
AGAGCGT	32720	40.99895	8
GATCGGA	32705	40.903984	1
GTGGTCG	32420	40.699783	41
GCGTCGT	32815	40.59009	11
GGTCGCC	32300	40.565857	43
GTAGATC	32895	40.54242	31
GTGTAGG	32995	40.223545	16
GTAGGGA	32980	40.195686	18
TGGTCGC	32805	40.181366	42
CTCGGTG	33170	40.11044	37
CGGTGGT	33085	40.10058	39
TCGGTGG	33285	40.090797	38
GAGCGTC	33435	40.084652	9
GAGTGTA	33440	39.995205	27
ATCTCGG	33320	39.77112	35