FastQCFastQC Report
Wed 4 May 2016
G128-M13_AGTTCC_BC8GTYANXX_L001_001.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG128-M13_AGTTCC_BC8GTYANXX_L001_001.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18646381
Sequences flagged as poor quality0
Sequence length50
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTCGTAT2248841.2060463636348522TruSeq Adapter, Index 14 (97% over 44bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1316680.7061316616881314No Hit
GTCTTTTTGTTATTTTGTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT355840.19083595899922887No Hit
GGAGCATACTGGTGCTGATTATGAAGAGGAGTTCAAAGGGGGCGCTGTCT219110.11750805692536262No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA308900.042.2710151
CTCGTAT292550.041.96409644
ACGTCTG316000.041.95508215
TCCGTAT270300.041.84662637
ACACGTC317100.041.8245113
CGTCTGA316800.041.8166716
CGTATCT267800.041.44222639
GTTCCGT300800.041.356235
CACGTCT324000.041.05958614
TATCTCG273550.040.9077141
CACACGT320800.040.8570812
ATCGGAA321300.040.683882
AGTTCCG308100.040.4733934
CCGTATC274250.040.45953438
TCTCGTA281000.040.21915443
TTCCGTA311250.039.87754436
TCGGAAG330950.039.7350773
CGGAAGA331250.039.2556884
GCACACG337300.038.8834811
GAACTCC349900.037.76204321
Produced by FastQC (version 0.11.2)