FastQCFastQC Report
Wed 4 May 2016
G128-M12_CTTGTA_BC8GTYANXX_L001_001.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG128-M12_CTTGTA_BC8GTYANXX_L001_001.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22913957
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG1795370.7835268260300916Illumina Single End PCR Primer 1 (100% over 50bp)
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN290260.1266738870113093No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCTCG229500.039.43494834
GTAGATC233250.039.02276231
TCTCGGT232950.038.87915436
CGTGTAG230150.038.8186615
ATCTCGG232900.038.79315635
TCGTGTA229200.038.77909514
CGTCGTG233850.038.42109712
GCGTCGT233950.038.40635311
GTAGGGA234300.038.093718
GATCGGA237750.037.6763531
GTCGTGT239500.037.52356713
GTGGTCG239900.037.47933641
GAGTGTA243450.037.17355727
AGAGCGT242350.037.1462678
TGTAGGG243150.037.01473617
ATCGGAA242100.036.844412
AGTGTAG245850.036.8266328
AGCGTCG245700.036.7054310
GTGTAGG245100.036.702316
TGGTCGC245100.036.65717742
Produced by FastQC (version 0.11.2)