FastQCFastQC Report
Wed 4 May 2016
G128-M12_CTTGTA_BC8GTYANXX_L001_001.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG128-M12_CTTGTA_BC8GTYANXX_L001_001.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22913957
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGC1503400.656106668961629TruSeq Adapter, Index 12 (100% over 50bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT283150.12357097466840844No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA206100.041.823611
TCGTATG211300.040.5078443
CGTATGC212550.040.26009444
CTCGTAT207650.039.6867842
ATCGGAA216850.039.685662
CGTCTGA220550.039.6037316
ACACGTC221800.039.49398813
ACGTCTG223850.039.23464215
TCTCGTA204350.037.99751341
CACGTCT232500.037.83078414
CACACGT228250.037.77200312
AATCTCG210900.036.66186539
ATCTCGT214150.036.15632640
CGGAAGA241400.035.6438334
TCGGAAG248300.035.230363
GCACACG246950.034.8133911
GTAATCT225750.034.1871937
TAATCTC227900.033.96582438
CTTGTAA253650.033.23554234
ACCTTGT254550.033.2238532
Produced by FastQC (version 0.11.2)