FastQCFastQC Report
Thu 24 Mar 2016
G126-G127-M1M20-NEBNext14_AGTTCC-_AC8KUAANXX_L005_001.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG126-G127-M1M20-NEBNext14_AGTTCC-_AC8KUAANXX_L005_001.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14649498
Sequences flagged as poor quality0
Sequence length50
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG728800.4974914498776682Illumina Single End PCR Primer 1 (100% over 50bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA558000.3809004240281817No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT264250.18038160761549646No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN161380.11016077137933328No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGT314150.042.37327611
CGTCGTG328450.042.24817312
GTCGTGT326350.041.6372613
TCGTGTA325250.041.4817614
GTAGGGA330450.041.42157718
AGCGTCG308250.041.3361610
CGTGTAG329150.041.31583815
GAGCGTC310100.041.0956659
AGAGCGT317600.040.919378
GATCTCG201050.040.8133934
TGTAGGG346600.040.67630417
TAGGGAA331650.040.645319
GTCGCCG170550.040.6017944
ATCTCGG201700.040.3357535
GATCGGA335150.040.288851
TCTCGGT199900.040.08738736
ATCGGAA335900.039.997912
GGTCGCC176650.039.9723443
GTGTAGG349800.039.74442716
AAGAGCG321900.039.2603847
Produced by FastQC (version 0.11.2)