FastQCFastQC Report
Sun 3 Apr 2016
G126-G127-M21M42-NEBNext7_CAGATC-_AC8KUAANXX_L006_001.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG126-G127-M21M42-NEBNext7_CAGATC-_AC8KUAANXX_L006_001.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16984775
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGC884180.5205721006018625TruSeq Adapter, Index 7 (100% over 50bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT256590.15107059116179047No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA238010.14013138237038758No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC217800.040.8972344
TCGTATG219250.040.48631743
ACGTCTG210450.039.94521315
ACACGTC213600.039.64464613
CTCGTAT218000.039.5380742
CGTCTGA209850.039.36693616
GATCGGA149600.039.0799451
ATCGGAA153800.038.533782
CACGTCT224550.038.4665314
CGGAAGA232550.038.21354
CACACGT222650.037.93520712
TCTCGTA206800.037.39351741
GCACACG230750.036.70406711
AGTCACC253700.035.96109428
TCGGAAG174300.035.4780433
CATCTCG218850.035.3046739
ATCTCGT221150.035.10656440
ACCAGAT240300.034.98152532
AGAGCAC261950.033.940898
GAACTCC259200.033.72453321
Produced by FastQC (version 0.11.2)