FastQCFastQC Report
Sun 3 Apr 2016
G126-G127-M21M42-NEBNext1_ATCACG-_AC8KUAANXX_L006_001.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG126-G127-M21M42-NEBNext1_ATCACG-_AC8KUAANXX_L006_001.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12890883
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAAGCAGTAGCAGAGTGATCGGAAGAGCACACGTCTGAACTCCAGTCCCA667750.5180017536424774TruSeq Adapter, Index 1 (97% over 34bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGC505170.3918816112131341TruSeq Adapter, Index 1 (100% over 50bp)
GAGCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGC148420.11513563500653913TruSeq Adapter, Index 1 (98% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGATCG121850.041.1987415
TGATCGG130850.041.02367416
TCGTATG134850.040.12160543
CGTATGC136600.039.88131344
ATCACGA137050.039.36567734
CTCGTAT133500.038.86355642
ACGATCT120250.038.6827937
TCACGAT126800.038.12480535
AGTGATC143350.038.01474414
CACGATC124050.037.3917436
GAGTGAT141350.036.60552613
CATCACG148850.036.52572333
TCTCGTA129500.036.3614441
AGTAGCA158850.035.981526
CGATCTC132250.035.438938
GCAGTAG167450.034.9721954
TAGCAGA149650.034.9230968
GATCTCG134700.034.7943239
AGAGTGA153100.033.52101512
GTAGCAG158150.033.5161257
Produced by FastQC (version 0.11.2)