FastQCFastQC Report
Mon 4 Apr 2016
G126-G127-M21M42-NEBNext8_ACTTGA-_AC8KUAANXX_L006_001.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG126-G127-M21M42-NEBNext8_ACTTGA-_AC8KUAANXX_L006_001.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11716980
Sequences flagged as poor quality0
Sequence length50
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGC754730.6441335565990555TruSeq Adapter, Index 8 (100% over 50bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT719150.6137673700902452No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA625640.5339601160025877No Hit
GAGCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGC221760.1892637864022982TruSeq Adapter, Index 8 (98% over 50bp)
GTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT130130.11106104132634861No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC177450.041.18528744
TCGTATG177050.041.1665443
CTCGTAT170700.040.53339442
AATCTCG156050.039.84347539
TCTCGTA161750.038.86042841
ACGTCTG181550.037.57120515
CGTCTGA178600.037.4397816
TTGAATC166300.037.21577536
ACACGTC185400.037.18290313
GAATCTC167600.036.7040238
TGAATCT170150.036.28320737
CACGTCT192050.036.26225314
CACTTGA198300.036.21226533
GTCACAC206100.035.88757329
CGGAAGA198800.035.7959254
CACACGT193400.035.41324212
ACTTGAA204500.035.039134
GCACACG196500.035.0185111
GAGCGGA43350.034.939551
ACACTTG210300.034.7211432
Produced by FastQC (version 0.11.2)