FastQCFastQC Report
Mon 4 Apr 2016
G126-G127-M21M42-NEBNext7_CAGATC-_AC8KUAANXX_L006_001.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG126-G127-M21M42-NEBNext7_CAGATC-_AC8KUAANXX_L006_001.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16984775
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA212120.12488831909754472No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGT171050.041.15062311
CGTCGTG175800.040.53693412
TCGTGTA186050.039.78637714
CGTGTAG184550.039.47286615
GTCGTGT180300.039.29244213
GATCGGA196600.039.2807851
AGCGTCG175800.039.07701510
GTAGGGA180800.039.0294318
GAGCGTC178400.038.7170569
AGAGCGT185400.038.2800038
TGTAGGG191550.038.21461517
ATCGGAA201700.038.0375562
GATCTCG114500.037.77073334
GTGTAGG197900.036.63524616
GTAGATC133750.036.63059631
TAGGGAA191400.036.53030819
ATCTCGG123300.036.49814235
GAGTGTA170700.036.3429927
TCTCGGT120050.036.2677736
AAGAGCG190750.036.078037
Produced by FastQC (version 0.11.2)