FastQCFastQC Report
Mon 4 Apr 2016
G126-G127-M21M42-NEBNext6_GCCAAT-_AC8KUAANXX_L006_001.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG126-G127-M21M42-NEBNext6_GCCAAT-_AC8KUAANXX_L006_001.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16203722
Sequences flagged as poor quality0
Sequence length50
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA263320.16250587365051067No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGT158450.041.59389511
CGTCGTG162750.040.9112812
GTCGTGT166800.039.7836413
CGTGTAG173450.039.7684715
TCGTGTA175400.039.75858314
GATCGGA179250.039.57071
AGCGTCG164700.039.17603310
GTAGGGA167400.039.12318418
GAGCGTC166100.038.964999
ATCGGAA182850.038.455232
AGAGCGT173150.038.0456928
GATCTCG106500.037.65200834
TGTAGGG179900.037.63739817
ATCTCGG110550.037.6182535
TCTCGGT111300.036.8078636
GTAGATC125150.036.58737631
GTGTAGG184650.036.38530316
TAGGGAA178200.036.23036219
TCGGAAG199650.036.154153
GTCGCCG87000.036.13409844
Produced by FastQC (version 0.11.2)