FastQCFastQC Report
Mon 4 Apr 2016
G126-G127-M21M42-NEBNext5_ACAGTG-_AC8KUAANXX_L006_001.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG126-G127-M21M42-NEBNext5_ACAGTG-_AC8KUAANXX_L006_001.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9682520
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGC507610.5242540165163614TruSeq Adapter, Index 5 (100% over 50bp)
GAGCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGC152350.1573454018168824TruSeq Adapter, Index 5 (98% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC128950.040.8838744
TCGTATG128700.040.84366243
CTCGTAT122650.039.827842
ACACGTC124200.039.65587213
ACGTCTG124550.039.04946515
CGTCTGA123250.038.8182516
GATCGGA87800.038.4332851
CACGTCT130350.038.19026614
CACACGT128550.038.0407212
TCTCGTA115200.037.9359341
CGGAAGA135950.037.8194
GCACACG132200.037.12625511
ATCGGAA92300.037.0247232
AGTGATC116750.036.3202436
GATCTCG119600.036.22691739
ATCTCGT123600.035.21467240
GTCACAC152500.034.6846329
TCGGAAG102550.034.5678143
GAACTCC147750.034.46933421
AGAGCAC152900.033.8795858
Produced by FastQC (version 0.11.2)