FastQCFastQC Report
Mon 4 Apr 2016
G126-G127-M21M42-NEBNext4_TGACCA-_AC8KUAANXX_L006_001.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG126-G127-M21M42-NEBNext4_TGACCA-_AC8KUAANXX_L006_001.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8042616
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGT75000.040.6344711
CGTCGTG76450.040.20615412
GATCGGA81950.039.7939381
CGTGTAG78950.039.46536315
AGCGTCG76450.039.17642210
TCGTGTA80350.039.1117414
GAGCGTC77700.038.546179
GTAGGGA78000.038.5065318
ATCGGAA84400.038.211322
GATCTCG49400.038.1305634
AGAGCGT79600.038.010138
GTCGTGT80200.037.66541313
ATCTCGG52100.036.9645535
TGTAGGG83500.036.8115817
TCGGAAG91600.035.7822573
TCTCGGT52100.035.7737236
TAGGGAA83350.035.76948519
GTAGATC59150.035.47957631
GTGTAGG86800.035.46375316
AAGAGCG83300.035.3715557
Produced by FastQC (version 0.11.2)