FastQCFastQC Report
Mon 4 Apr 2016
G126-G127-M21M42-NEBNext12_CTTGTA-_AC8KUAANXX_L006_001.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG126-G127-M21M42-NEBNext12_CTTGTA-_AC8KUAANXX_L006_001.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11231164
Sequences flagged as poor quality0
Sequence length50
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1760951.5679140648288994No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT807710.7191685563491015No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGT203100.042.7049211
CGTCGTG206300.042.54124512
TCGTGTA218450.042.0150914
CGTGTAG217550.042.0031215
AGCGTCG203300.041.82197610
GTCGTGT209350.041.75998713
GAGCGTC204050.041.635929
GATCGGA231650.041.6239361
AGAGCGT209500.041.283718
GTAGGGA210150.041.1741118
ATCTCGG132200.041.12011735
GATCTCG128800.041.11918634
GTCGCCG102600.040.760644
ATCGGAA234500.040.7325332
TCTCGGT129650.040.71581636
TGTAGGG221400.040.4113217
GGTCGCC105050.040.0398543
GTGTAGG222450.039.81731816
TAGGGAA215450.039.63586419
GTAGATC152050.039.6109431
Produced by FastQC (version 0.11.2)