FastQCFastQC Report
Mon 4 Apr 2016
G126-G127-M21M42-NEBNext11_GGCTAC-_AC8KUAANXX_L006_001.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG126-G127-M21M42-NEBNext11_GGCTAC-_AC8KUAANXX_L006_001.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30039780
Sequences flagged as poor quality0
Sequence length50
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA457140.15217821169129733No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGT142550.038.3806711
CGTCGTG149600.036.99613612
TCGTGTA159850.035.9129714
CGTGTAG159950.035.7072415
GATCGGA168350.035.5843161
AGCGTCG152150.035.00646210
GTCGTGT157700.034.60618613
GTAGGGA161450.034.05344818
GAGCGTC159200.033.7048849
ATCGGAA177650.033.4505352
AGAGCGT167150.033.0615968
TGTAGGG176650.032.5156517
GATCTCG101150.032.2440434
GTGTAGG180750.031.52430516
GTAGATC119800.031.13014231
TAGGGAA176150.030.93348319
ATCTCGG109100.030.89161135
AAGAGCG179050.030.2752027
TCTCGGT110400.030.26816736
TCGGAAG205100.030.18893
Produced by FastQC (version 0.11.2)