FastQCFastQC Report
Mon 4 Apr 2016
G126-G127-M21M42-NEBNext11_GGCTAC-_AC8KUAANXX_L006_001.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG126-G127-M21M42-NEBNext11_GGCTAC-_AC8KUAANXX_L006_001.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30039780
Sequences flagged as poor quality0
Sequence length50
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATGC706060.23504166808145732TruSeq Adapter, Index 11 (100% over 50bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT654620.21791770778614225No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA357760.11909541281593941No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGGCTA176650.040.35196732
CGGCTAC185350.038.56458333
TCGTATG188600.037.85306543
CGTATGC190400.037.5876244
AGTCACG199100.036.92928728
CTCGTAT188350.036.8757442
TCACGGC198750.036.1307930
ACACGTC187650.035.94089513
GTCACGG204850.035.41970429
ACGTCTG188300.035.23216215
CGTCTGA186000.035.1232816
GATCGGA131900.034.311691
TCTCGTA183400.034.2133241
CACGGCT209600.034.15542631
CACACGT201800.033.4906912
CGGAAGA214850.033.1217964
CACGTCT206650.032.99865714
ATCGGAA139400.032.9380842
GCACACG207700.032.76988611
CATCTCG203000.030.92085339
Produced by FastQC (version 0.11.2)