FastQCFastQC Report
Mon 4 Apr 2016
G126-G127-M1M20-NEBNext15_ATGTCA-_AC8KUAANXX_L005_001.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG126-G127-M1M20-NEBNext15_ATGTCA-_AC8KUAANXX_L005_001.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12169082
Sequences flagged as poor quality0
Sequence length50
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG445870.3663957560644262Illumina Single End PCR Primer 1 (100% over 50bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA366810.30142783161457865No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT145970.11995152962236592No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN123370.10137987401186055No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGT195250.041.77335711
CGTCGTG205850.041.57588612
CGTGTAG205350.040.94721215
TCGTGTA205450.040.73476414
GTAGGGA207200.040.7334618
GTCGTGT206150.040.5358913
AGCGTCG193100.040.1660410
AGAGCGT197000.040.115238
GATCGGA201900.039.9065441
GATCTCG131550.039.80289534
TGTAGGG219350.039.71536617
GAGCGTC197000.039.5824289
ATCGGAA201500.039.507782
ATCTCGG129250.039.47715435
TAGGGAA210550.039.43552819
GTCGCCG110050.039.0195744
GTGTAGG220650.038.8077216
TCTCGGT130700.038.52141636
GGTCGCC112450.038.5201443
GTAGATC141050.038.08111631
Produced by FastQC (version 0.11.2)