FastQCFastQC Report
Mon 4 Apr 2016
G126-G127-M1M20-NEBNext13_AGTCAA-_AC8KUAANXX_L005_001.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG126-G127-M1M20-NEBNext13_AGTCAA-_AC8KUAANXX_L005_001.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences66913892
Sequences flagged as poor quality0
Sequence length50
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAACAATCTCGTAT3519400.5259595421530704TruSeq Adapter, Index 13 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT935250.040.0643844
AATCTCG830750.039.8716741
TCTCGTA880700.038.2876843
AGTCAAC1057200.038.11931634
GTCAACA1104600.036.4676435
CAGTCAA1115950.036.40209233
ACGTCTG1076550.036.15423215
CGTCTGA1063350.036.1064416
ATCTCGT929400.035.97361442
AACAATC976900.034.79915638
ACACGTC1163300.034.73882713
TCAACAA1141800.034.44529336
CACGTCT1182000.034.3248214
GAACTCC1177400.033.80118621
CAACAAT1066300.033.0508337
CACACGT1235350.032.99238212
AGTCACA1271950.032.95271328
ACAGTCA1233500.032.88681832
CAGTCAC1274850.032.86570727
CAATCTC1040300.032.5050140
Produced by FastQC (version 0.11.2)