RXR_TCPO_Reps_Combined.R2.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	RXR_TCPO_Reps_Combined.R2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	30129410
Sequences flagged as poor quality	0
Sequence length	50
%GC	44

Per base quality graph

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG	487789	1.6189795950202808	Illumina Single End PCR Primer 1 (100% over 50bp)
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN	49860	0.16548614791992278	No Hit

Adapter graph

Kmer graph

Sequence	Count	Obs/Exp Max	Max Obs/Exp Position
CGTCGTG	92010	43.305508	12
AGCGTCG	91590	43.26902	10
GCGTCGT	92075	43.243107	11
GATCGGA	92450	43.1704	1
GTCGCCG	58655	43.07954	44
ATCGGAA	92265	43.051342	2
TCGTGTA	91910	43.024513	14
GTCGTGT	92720	43.011097	13
GAGCGTC	92515	42.898537	9
GATCTCG	67675	42.866447	34
CGTGTAG	92870	42.852867	15
TGGTCGC	57780	42.851944	42
GTGGTCG	60735	42.764412	41
AGAGCGT	93290	42.688923	8
TCGGAAG	94080	42.601902	3
ATCTCGG	68970	42.538353	35
AAGAGCG	93350	42.44291	7
TCTCGGT	67405	42.401768	36
CGGAAGA	93290	42.393574	4
TCGGTGG	70870	42.388985	38