FastQCFastQC Report
Fri 19 May 2017
CEBPb_All_Reps_Combined.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameCEBPb_All_Reps_Combined.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences92129597
Sequences flagged as poor quality0
Sequence length50
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATGC1340830.1455373781782634TruSeq Adapter, Index 10 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTGA1170500.041.38020716
ACGTCTG1183050.041.0381615
GATCGGA1177800.040.6897771
CGTATGC514500.040.64543544
CCGTATC347000.039.99103538
ATCGGAA1206000.039.8382
TCGTATG528500.039.64078543
ACACGTC1227300.039.60692613
TCGGAAG1221000.039.3176963
TCCGTAT363900.039.08865737
CGTATCT356900.039.03554539
CGGAAGA1231350.038.830074
AGTTCCG408350.038.7639634
GCACACG1278450.037.99046311
CACACGT1282750.037.97315212
CACGTCT1292250.037.67243214
GTTCCGT434250.036.2808435
GAACTCC1332450.036.25954421
CGACGCT70800.035.80498517
TTCCGTA442050.035.54129836
Produced by FastQC (version 0.11.2)