FastQCFastQC Report
Tue 2 Aug 2016
G124-G125-M1M19-NEBNext4_TGACCA-_AC8KUAANXX_L007_001.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG124-G125-M1M19-NEBNext4_TGACCA-_AC8KUAANXX_L007_001.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17098826
Sequences flagged as poor quality0
Sequence length50
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN172120.10066188169877861No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCGTG50650.040.2850912
GTCGCCG31400.040.28059844
AGCGTCG51400.040.12821210
GCGTCGT51200.039.9836611
GAGCGTC53800.038.2968569
TGGTCGC32900.037.44153642
GTCGTGT55200.037.31921413
CGTGTAG54700.037.127715
TCGTGTA54500.037.10561414
GATCGGA55050.037.015031
GTGGTCG34850.036.98761741
GGTCGCC34100.036.76869643
GATCTCG39200.036.63434
ATCGGAA55650.036.462052
AGAGCGT56950.036.0631648
AAGAGCG57250.035.7587
TCGGAAG57450.035.4115143
TCGGTGG43050.034.79292738
TCTCGGT41000.034.00834736
CGGAAGA59300.033.718154
Produced by FastQC (version 0.11.2)