FastQCFastQC Report
Tue 2 Aug 2016
G124-G125-M1M19-NEBNext4_TGACCA-_AC8KUAANXX_L007_001.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG124-G125-M1M19-NEBNext4_TGACCA-_AC8KUAANXX_L007_001.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17098826
Sequences flagged as poor quality0
Sequence length50
%GC40

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGC420540.2459467100255889TruSeq Adapter, Index 4 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC56300.039.5642844
CTCGTAT54500.038.89571842
TCGTATG57550.038.8212643
GATCGGA57400.038.655361
TCTCGTA49350.038.5893341
CGTCTGA59800.037.9778316
ACGTCTG60700.037.34222415
ATCGGAA60250.037.005642
ACACGTC62400.036.3599413
AATCTCG53150.035.58210839
TCGGAAG64000.035.3180163
CGGAAGA64200.035.0024954
ATCTCGT54550.034.83016240
GCACACG70450.032.030611
CACACGT70950.032.00041612
CACGTCT80400.028.32913814
ACTGACC81950.027.50418332
GACCAAT72250.027.36266335
ACCAATC70800.026.9291336
GAACTCC86100.026.73327621
Produced by FastQC (version 0.11.2)