FastQCFastQC Report
Thu 6 Apr 2017
G124_G125_M20M21.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG124_G125_M20M21.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35148509
Sequences flagged as poor quality0
Sequence length50
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG1121090.3189580530997773Illumina Single End PCR Primer 1 (100% over 50bp)
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN525990.1496478840681407No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGT205400.041.25161711
CGTCGTG206150.041.17299312
AGCGTCG205350.041.120310
GTCGCCG140000.040.3460144
GATCGGA213950.040.02441
TGGTCGC137700.039.7747142
GTCGTGT215100.039.67038713
TCGTGTA214450.039.47738614
GAGCGTC214350.039.4652069
CGTGTAG215700.039.42876415
GATCTCG162850.039.38084434
ATCGGAA216150.039.336332
GTGGTCG147850.038.9782441
GGTCGCC147950.038.28183743
AGAGCGT222100.038.1865548
ATCTCGG169800.038.1708235
TCTCGGT166800.037.9623636
TCGGAAG226650.037.745623
TCGGTGG175700.037.59243438
AAGAGCG225350.037.550227
Produced by FastQC (version 0.11.2)