FastQCFastQC Report
Thu 6 Apr 2017
G124-G125-M20M38-NEBNext21_GTTTCG-_AC8KUAANXX_L008_001.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG124-G125-M20M38-NEBNext21_GTTTCG-_AC8KUAANXX_L008_001.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20790793
Sequences flagged as poor quality0
Sequence length50
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTTCGGAATCTCGTAT960600.4620314386276656TruSeq Adapter, Index 21 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTCG114900.042.0168733
CTCGTAT116200.040.2569944
TCGGAAT111700.040.22772237
GTTTCGG120400.040.09768734
CGGAATC110150.039.9950938
TCTCGTA110500.039.84601243
TTTCGGA121600.039.61145435
GATCGGA128200.039.5733871
CGTCTGA128900.039.5058516
TTCGGAA122100.039.30513836
AATCTCG111450.039.290941
ATCGGAA130100.038.9452
ACACGTC130450.038.86739713
ACGTCTG130300.038.84483315
GTCACGT127100.038.39835429
AGTCACG129150.038.21753728
ACGTTTC128200.037.89832332
ATCTCGT116650.037.85874642
TCGGAAG137500.036.7370953
CGGAAGA137150.036.622494
Produced by FastQC (version 0.11.2)