FastQCFastQC Report
Mon 3 Apr 2017
G124-G125-M1M19-NEBNext20_GTGGCC-_AC8KUAANXX_L007_001.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG124-G125-M1M19-NEBNext20_GTGGCC-_AC8KUAANXX_L007_001.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19837857
Sequences flagged as poor quality0
Sequence length50
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT11550.019.03774544
TCTCGTA11200.018.4552743
TATCTCG12150.016.10817141
CGTCTGA18450.013.47905816
ACACGTC20000.012.76448413
GATCGGA19000.012.5200771
ATCTCGT16450.012.43161842
ATCGGAA19800.011.9930532
ACGTCTG21550.011.84644915
AGTCACG20500.011.2633628
ACGTGGC23050.010.01687532
GTCACGT21600.09.77315129
CGTGGCC22950.09.58147333
TCGGAAG26050.09.3688063
CACGACG3604.363237E-68.55860615
CACGTCT34100.08.39004414
ACGACGC3809.021427E-68.10815216
CGGAAGA29900.08.0153844
CGACGCT4551.5147652E-67.739022717
GCACACG38050.07.16711511
Produced by FastQC (version 0.11.2)