FastQCFastQC Report
Mon 1 May 2017
G124_G125_M13M14M15.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG124_G125_M13M14M15.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences58498586
Sequences flagged as poor quality0
Sequence length50
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAACAATCTCGTAT1900240.3248352019995834TruSeq Adapter, Index 13 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT897150.042.3697944
TCTCGTA829800.041.8302443
CGTCTGA978650.041.72963316
ACGTCTG988550.041.39409315
ATCTCGT840500.041.20876342
CCGTATC332950.041.10793738
GATCGGA989350.040.7688831
AATCTCG513850.040.48032441
CGTATCT339100.040.47263739
TCCGTAT347700.040.31275637
AGTTCCG385600.040.2953634
ATCGGAA1011550.039.8774642
ACACGTC1028150.039.83173813
TCGGAAG1021600.039.474073
TATCTCG351050.039.326741
CGGAAGA1025500.039.0838174
GTTCCGT398400.038.70968635
GCACACG1060150.038.61152311
CACGTCT1064700.038.526514
CACACGT1066400.038.4216712
Produced by FastQC (version 0.11.2)