FastQCFastQC Report
Mon 1 May 2017
G124_G125_M10M11M12.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG124_G125_M10M11M12.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences53115842
Sequences flagged as poor quality0
Sequence length50
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATGC1340800.25242939761738126TruSeq Adapter, Index 10 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC654600.042.01239844
TCGTATG660050.041.6270943
CTCGTAT639650.041.6036442
TCTCGTA592750.041.60289841
CGTCTGA680700.041.37589316
ACGTCTG685700.041.16084315
GATCGGA684250.040.9432371
ATCTCGT604950.040.6802940
ATCGGAA701850.039.993292
ACACGTC710000.039.79855313
AATCTCG308100.039.44153639
TCGGAAG710900.039.3819473
ACGGCTA176600.039.0234932
CGGCTAC176650.038.93779433
CGGAAGA717100.038.906544
CACGTCT741450.038.17872614
CACACGT744000.038.06597512
GCACACG742850.038.04167611
TATCTCG173150.036.76721239
TCACGGC189350.036.4656430
Produced by FastQC (version 0.11.2)