FastQCFastQC Report
Mon 1 May 2017
G124-G125-M1M19-NEBNext14_AGTTCC-_AC8KUAANXX_L007_001.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG124-G125-M1M19-NEBNext14_AGTTCC-_AC8KUAANXX_L007_001.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17550119
Sequences flagged as poor quality0
Sequence length50
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTCGTAT2904501.6549745332211139TruSeq Adapter, Index 14 (97% over 44bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT348900.042.46512644
CCGTATC318200.042.19006338
CGTCTGA375700.041.94895616
TCTCGTA324300.041.93120243
TCCGTAT329050.041.8948937
CGTATCT321600.041.8807839
ACGTCTG378400.041.76014315
AGTTCCG364750.041.74196234
ATCTCGT327150.041.42476342
GTTCCGT365650.041.4119935
TATCTCG326200.041.34411641
TTCCGTA366200.041.19967336
GATCGGA383850.040.760211
ATCGGAA390400.040.0937772
ACACGTC396150.039.95557813
TCGGAAG393650.039.6845633
CGGAAGA395700.039.3067174
GAACTCC403550.039.1000221
GTATCTC345850.039.0523140
CACACGT406350.038.96582812
Produced by FastQC (version 0.11.2)