FastQCFastQC Report
Mon 1 May 2017
G124-G125-M1M19-NEBNext11_GGCTAC-_AC8KUAANXX_L007_001.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG124-G125-M1M19-NEBNext11_GGCTAC-_AC8KUAANXX_L007_001.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15002469
Sequences flagged as poor quality0
Sequence length50
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATGC1287190.8579854422628702TruSeq Adapter, Index 11 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC161850.041.99432844
CGGCTAC162850.041.76596533
ACGGCTA164450.041.53330232
CTCGTAT159350.041.45429642
TCTCGTA145050.041.44968841
TCGTATG163900.041.4170743
CGTCTGA170650.040.9957616
TCACGGC168150.040.82850330
GTCACGG169050.040.58471729
GATCGGA171650.040.568321
ACGTCTG173250.040.50755315
ATCTCGT148050.040.49094840
CACGGCT170100.040.2313131
CATCTCG148550.040.20662739
AGTCACG171550.040.17380528
ATCGGAA175200.039.8209272
ACACGTC178750.039.21166213
TCGGAAG178650.038.9778183
CGGAAGA179500.038.511514
GCACACG187400.037.43784311
Produced by FastQC (version 0.11.2)