FastQCFastQC Report
Mon 1 May 2017
G124_G125_M7M8M9.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG124_G125_M7M8M9.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences44825146
Sequences flagged as poor quality0
Sequence length50
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN476390.10627740063579491No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCGTG116800.040.1780712
GCGTCGT118550.039.53329511
GTCGCCG73000.039.5032444
AGCGTCG118900.039.4725110
GATCGGA131700.036.9900171
GAGCGTC126450.036.976449
CGTGTAG129900.036.48612615
GTCGTGT129700.036.36533413
TCGTGTA129600.036.18405514
ATCGGAA135500.035.940642
TGGTCGC78500.035.78286442
GGTCGCC81350.035.23223543
GTGGTCG82950.035.16240341
GATCTCG91250.035.1144134
AGAGCGT134900.034.9705548
AAGAGCG137000.034.723047
TCGGAAG141250.034.3699953
TCGGTGG103500.032.87475638
CGGAAGA145750.032.8009874
CGGTGGT97750.032.17598339
Produced by FastQC (version 0.11.2)