FastQCFastQC Report
Mon 1 May 2017
G124_G125_M4M5M6.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG124_G125_M4M5M6.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences47333156
Sequences flagged as poor quality0
Sequence length50
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN473580.10005248752058704No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGCCG93650.040.02373544
AGCGTCG150250.039.9975310
CGTCGTG149650.039.99257312
GCGTCGT150550.039.69811611
GATCGGA161150.037.631161
GTCGTGT160650.037.3872613
TGGTCGC97700.037.30663342
GAGCGTC160750.037.206729
GATCTCG112450.037.17772734
CGTGTAG161750.037.05539315
GTGGTCG103150.036.97733741
ATCGGAA164750.036.706732
TCGTGTA162250.036.61945314
GGTCGCC101600.036.4373443
TCGGAAG170900.035.583223
AGAGCGT169550.035.4058238
AAGAGCG171700.035.0258757
TCGGTGG128200.034.1757838
TCTCGGT121800.033.98247536
CGGAAGA177050.033.756134
Produced by FastQC (version 0.11.2)