FastQCFastQC Report
Mon 1 May 2017
G124_G125_M4M5M6.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG124_G125_M4M5M6.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences47333156
Sequences flagged as poor quality0
Sequence length50
%GC40

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC165100.039.54203444
TCGTATG166650.039.1891143
CTCGTAT160450.039.03143742
GATCGGA170050.038.9365581
TCTCGTA146800.038.90258841
CGTCTGA173950.038.3594616
ATCGGAA177400.037.455782
ACGTCTG180600.037.03229515
ACACGTC184050.036.34993413
ATCTCGT158150.036.0411340
TCGGAAG187650.035.561753
CGGAAGA190500.034.9489254
CGCCAAT64650.033.3669933
ACGCCAA66250.032.6937732
GCACACG205500.032.58844411
CACACGT208800.032.08400712
GTCACGC68250.031.67144229
GATCTCG62500.029.8365439
CACGTCT225850.029.77830914
AGTCACG76150.028.67516928
Produced by FastQC (version 0.11.2)