FastQCFastQC Report
Wed 16 Mar 2016
G126-G127-M1M20-NEBNext23_GAGTGG-_AC8KUAANXX_L005_001.R1_trimmed.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG126-G127-M1M20-NEBNext23_GAGTGG-_AC8KUAANXX_L005_001.R1_trimmed.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7634955
Sequences flagged as poor quality0
Sequence length20-50
%GC59

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCC86500211.329497030434364No Hit
CTTGGGGGGCCCAAGTCCTTCTGATCGAGGCC2428053.1801759145928172No Hit
CGGGGGGGGCCCAAGTCCTTCTGATCGAGGCC1961162.5686595402330465No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCC1375451.8015168393264926No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGC1134671.4861515228315032No Hit
GTTGGGGGCCCAAGTCCTTCTGATCGAGGCC799601.0472884254065675No Hit
CGTGTGGGGCCCAAGTCCTTCTGATCGAGGCC769191.0074584591526734No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGC677500.8873660683003372No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGC394370.5165321865027365No Hit
CTCGCCCGTCACGTTGAACGCACGTTCG383260.5019806927480254No Hit
CTCGCCCGTCACGTTGAACGCACGTTC381180.4992563806859372No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCT324330.4247962168735768No Hit
TACCCGTCGCCGCGCTCTCTCTCTCCCC289300.3789151344048524No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCAC258740.3388887033387885No Hit
CGGGGGGGGCCCAAGTCCTTCTGATCGAGGC254090.33279829416152423No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTT251220.3290392674220084No Hit
CGGGTGGGGCCCAAGTCCTTCTGATCGAGGCC233710.30610527501471846No Hit
GTTGGGGGCCCAAGTCCTTCTGATCGAGGC228680.2995171549799573No Hit
TGTGGGGGGCCCAAGTCCTTCTGATCGAGGCC218910.28672074688062993No Hit
GTGGGGGGGCCCAAGTCCTTCTGATCGAGGCC215620.2824116186670386No Hit
CGCGGGGGGCCCAAGTCCTTCTGATCGAGGCC200610.26275203979591233No Hit
CTAAACCATTCGTAGACGACCTGCTTC169660.22221480021820694No Hit
CGTGATCGTATAGTGGTTAGTACTCTGC162190.21243085257215008No Hit
ACAGAAAAGGCTGGTGAATATTCTGTGACG153160.20060367087952713No Hit
GCCGCCGGTGAAATACCACTACTCTCAT151280.1981413119003321No Hit
ATCACAAGAAAGACGTGGTCCTGACAGACA150750.1974471362306654No Hit
CGAGGGGGGCCCAAGTCCTTCTGATCGAGGCC147960.19379289072430683No Hit
ATCACAAGAAAGACGTGGTCCTGACAGAC145760.19091140681248284No Hit
TTTGTGACTCTAGATAACCTCGGGCCGATCGCAC139000.18205739261069645No Hit
GGGGGGGGCCCAAGTCCTTCTGATCGAGGCC137870.1805773576923505No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCAC132410.1734260385293692No Hit
GTTGGGGGGCCCAAGTCCTTCTGATCGAGGCC131510.1722472496563503No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATT127660.16720465281065833No Hit
TACCCGTCGCCGCGCTCTCTCTCTCCCCC126080.16513522345580295No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCA117750.1542248775533058No Hit
TCTGTGACGTATGATGGATTCAATACATTT111100.14551493754711062No Hit
NTTGGGGGGCCCAAGTCCTTCTGATCGAGGCC109790.1437991448541609No Hit
CCTGGGGGGCCCAAGTCCTTCTGATCGAGGCC109280.1431311644927835No Hit
CATGGGGGGCCCAAGTCCTTCTGATCGAGGCC108470.1420702545070665No Hit
TGGGGGGGGCCCAAGTCCTTCTGATCGAGGCC104210.13649065384144374No Hit
CTCGCCCGTCACGTTGAACGCACGTTCGT99250.12999421738569514No Hit
GTGGGGGGGCCCAAGTCCTTCTGATCGAGGC99070.12975845961109136No Hit
ACCCGTCGCCGCGCTCTCTCTCTCCCC98610.12915596752043726No Hit
TCTGTGACGTATGATGGATTCAATACATT96390.12624828830032397No Hit
NGTGGGGGGCCCAAGTCCTTCTGATCGAGGCC92840.12159862107897165No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGG90780.11890050432517282No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGT84990.11131696257541794No Hit
GCTGAGAAGACGGTCGAACTTGACTATC77550.10157230789179504No Hit
GGCCGCCGGTGAAATACCACTACTCTCAT76820.10061617913923526No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACGCG6550.0339.449744
TCACCCG3550.0338.545244
ATCACGC5100.0292.1347443
CGTAATA9900.0284.819437
CAGTCCC5350.0282.4058843
GTAATAT6500.0276.8737838
CCACGCG1050.0257.5361644
TCGTAAT10900.0255.8190336
TCACACG42950.0239.247844
GCCATAT13700.0225.9532830
AGTCACG5250.0223.19844
ATCGTTA4200.0221.4077135
GATCACG6050.0220.127542
CGGCCCC16250.0215.6558430
TAATATC8500.0214.9917939
ATCACCC4550.0207.537343
TCGTTAG4300.0204.9766236
GGCCAGA65500.0204.7166429
CCCGGGT11150.0203.0075834
GCCAGAT62950.0202.9624530
Produced by FastQC (version 0.11.2)